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DeCS
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Descriptor English:
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Ligase Chain Reaction
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Descriptor Spanish:
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Reacción en Cadena de la Ligasa
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Descriptor Portuguese:
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Reação em Cadeia da Ligase
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Synonyms English:
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Chain Reaction, Ligase
Chain Reactions, Ligase
Ligase Chain Reactions
Reaction, Ligase Chain
Reactions, Ligase Chain
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Tree Number:
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E05.393.620.311
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Definition English:
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A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured. |
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See Related English:
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Polymerase Chain Reaction
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History Note English:
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2001
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Allowable Qualifiers English:
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Record Number:
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35409
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Unique Identifier:
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D022521
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Occurrence in VHL:
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Similar:
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DeCS
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